Confocal z-stack and comparison of confocal vs STED images of Drosophila gut stem cells expressing chimeric (human extracellular, drosophila intracellular) epidermal growth factor receptor (EGFR) labeled with fluorescently conjugated (Alexa 594) anti-human EGFR antibody (Cetuximab). The STED technique is compatible with drosophila gut tissue and provides a significant resolution improvement over confocal, allowing the nanoscale determination of protein clustering in vivo.
Dr. Robert Fuentes
Image acquired on Leica SP8 Confocal, STED, FLIM system microscope, by Dr. Robert Fuentes (Dr. Chapkin’s group, Nutrition and Food Science) and sample prepared by Dr. Mohamed Mlih (Dr. Karpac’s group, Molecular and Cellular Medicine).